Viable, avirulent Leptospira interrogans serotype pomona vaccine: preservation in liquid nitrogen.
نویسنده
چکیده
By the addition of glycerol (10%, v/v), viability titers of serotype pomona vaccine were maintained within 1 to 2 logs (base 10) during controlled-rate freezing and storage in liquid nitrogen.
منابع مشابه
Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospira proteins in serovar Canicola; 60 of these proteins were common to Copenhageni and Pomona, 16 of...
متن کاملImmunoprotection of recombinant leptospiral immunoglobulin-like protein A against Leptospira interrogans serovar Pomona infection.
We previously reported the cloning and characterization of leptospiral immunoglobulin-like proteins LigA and LigB of Leptospira interrogans. LigA and LigB are conserved at the amino-terminal region but are variable at the carboxyl-terminal region. Here, we evaluate the potential of recombinant LigA (rLigA) as a vaccine candidate against infection by L. interrogans serovar Pomona in a hamster mo...
متن کاملCopurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C.
The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase C is responsible for hemolysis.
متن کاملFirst Genome Sequence of Leptospira interrogans Serovar Pomona, Isolated from a Bovine Abortion
Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary production. Here, we report the whole-genome sequence of Leptospira interrogans serovar Pomona strain AKRFB, isolated from a bovine abortion during a leptospirosis outbreak in Argentina.
متن کاملPlate assay for detection of Leptospira interrogans serovar pomona hemolysin.
A plate assay which utilizes the addition of a blood agar overlay for detection of hemolysin by colonies of Leptospira interrogans serovar pomona is described.
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ورودعنوان ژورنال:
- Applied microbiology
دوره 22 4 شماره
صفحات -
تاریخ انتشار 1971